🪟🔬 Functionalized coverslips

This protocol demonstrates how to create functionalized coverslips for inverted microscopy. These coverslips can be used for cell culture or Drosophila whole mounts. Pay attention to the coverslip thickness and that it matches your objective/settings.

Always label batches with coverslip thickness.

This protocol assumes the etching and coating is done in Coplin jars.

🚛 Reagents needed 📦 :
Item Item article number Distributor Vendor
APTES 440140 Merck SigmaAldrich
UltraPure™ DNase/RNase-Free Distilled Water 10977035 ThermoFisher Invitrogen
Glacial acetic acid A6283-100ML Merck SigmaAldrich
Cover glass Borosilicate 631-0124 VWR VWR

 Clean coverslips in 1M KOH in for 20min.
   (make 1 L of 1M KOH by dissolving 56.11 g of KOH in 1 L of deionized water).

 Rinse three times in nuclease-free water.

 Rinse one time in 100% methanol.

 Let the coverslip dry from the methanol and then immerse in APTES, (3-aminopropyl)triethoxysilane, for 2 min:

Reagent Amount Volume Final concentration
100% Methanol 55.2 mL
Acetic acid 3 mL  5% vol/vol
APTES 1800 µl 3% vol/vol
60 mL

 Rinse three times in nuclease-free water.

 Dry the coverslips in a dark and desiccated environment in room temperature.
Store dry or use immediately.