DSS (disuccinimidyl suberate) crosslinking in situ
This protocol describes how to use DSS to crosslink primary amines in situ.
|1xPBS pH 8
2️⃣ Add to the sample and incubate for 1h at room temperature.
3️⃣Quench the reaction by aspirating and adding 1 M Tris pH 8.0 and incubate for 30 min.
5️⃣The sample is now ready.