3M Sodium acetate buffer stock buffer

Formula for precipitation. Not for enzymology or chromatography.

Use either Anhydrous or Trihydrate.

Reagent Amount Fill with water
Sodium acetate Anhydrous (82.03 g/mol)
410 mg
49 ml
Sodium acetate Trihydrate (136.08 g/mol)
680 mg
49 ml

Adjust pH with Acetic acid (glacial, molecular weight: 60.05 g/mol) by adding 290 ul observe the pH and if 5.2 to 5.5. pH fill up to 50 ml.